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In its recently re-implemented version 2.0, EpiToolKit provides a range of new functionality and for the first time allows combining tools into complex workflows. It offers an array of immunoinformatics tools covering MHC genotyping, epitope and neo-epitope prediction, epitope selection for vaccine design, and epitope assembly. 31: 359-362).ĮpiToolKit - is a virtual workbench for immunological questions with a focus on vaccine design. Nucleic Acids Research 34: W202-W209.), and SDAP - Structural Database of Allergenic Proteins ( Reference: Ivanciuc, O. BMC Bioinformatics 14(Suppl 6): S4), AlgPred - prediction of allergenic proteins and mapping of IgE epitopes ( Reference: Saha, S.
#Molecular weight calculation tool pro
Biotech Software and Internet Report 2:198-200).Īntigenicity and allergenicity: a good place to start would be The Immune Epitope Database ( IEDB)Ībie Pro Peptide Antibody Design (Chang Bioscience)Īllergenicity servers: AllerTOP ( Reference: Dimitrov, I.
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Lyu Lab., National Tsing-Hua University, Taiwan) - calculates the theoretical protein melting temperature.Ĭomputation of size of DNA and Protein Fragments from Their Electrophoretic Mobility ( Reference: Raghava, G. Putnam, The Scripps Research Institute, U.S.A.) - calculates mass, pI, charge at a given pH, counts amino acid residues etc. Web Server issue ).Ĭomposition/Molecular Weight Calculation (Georgetown University Medical Center, U.S.A.) - the only problem with this site is that when run in batch mode it does not identify the sequence by name, merely sequential numberīatch Protein Isoelectric Point determination - part of the Sequence Manipulation Suite or ENDMEMOīatch Protein Molecular Weight determination - part of the Sequence Manipulation Suite or ENDMEMO The prediction accuracy (RMSD) of IPC 2.0 for proteins and peptides outperforms previous algorithms. Isoelectric Point Calculator 2.0 (IPC 2.0) - is a server for the prediction of isoelectric points and p K a values using a mixture of deep learning and support vector regression models. You can also specify post-translational modifications, such as N- and C- terminal modifications and positioning of disulfide bridges, to obtain more accurate outputs.
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Peptide Molecular Weight Calculator (GenScript) - the online calculator determines the chemical formula and molecular weight of your peptide of interest. Biochemistry-online (Vitalonic, Russia) gives one % composition, molecular weight, pI, and charge at any desired pH. Mass, pI, composition and mol% acidic, basic, aromatic, polar etc. If you want a plot of the relationship between charge and pH use ProteinChemist () or JVirGel Proteomic Tools (PRODORIC Net, Germany). Isoelectric Point - Compute pI/Mw tool (ExPASy, Switzerland). If you are specifically interested in antibodies I would recommend that you visit " The Antibody Resource Page."Īmino acid composition & Mass – ProtParam (ExPASy, Switzerland) For the biochemical properties of amino acids see PROWL, Amino Acid Hydrophobicity and Amino Acid Chart and Reference Table (GenScript). You can also calculate the money you can save when you re-use rainwater that falls instead of tap-water ( precipitation calculator) and the energy cost when you use a boiler to heat water ( energy cost).Online Analysis Tools - Protein ChemistryīACKGROUND INFORMATION: You might want to consult Robert Russell's Guide to Structure Prediction. The other conversion programs have more units to convert and they have some useful back ground information. There is a small overview of the units that are mostly used in the industry in the process flow calculator. With the conversion calculators you can convert the units that are most commonly used. This may be useful, because most American and British use American or British units and European use the standard metric units. With these calculators you can calculate Anglo-American units to the standards units (SI-units). Ion exchange calculator for more than one chemical element, selectivity factor and separation factor Ion exchange calculator for one chemical element Hardness Converter and Hardness Calculatorĭesign Characteristics for a Municipal Wastewater Treatment Plant CalculationĮvaporation of water (lake en pan evaporation)